Browsing by Author "Suleiman Kafilat Olayemi"

Now showing 1 - 3 of 3

Investigation of Bacteria Biostimulation Strategy for Heavy Metal Bioremediation in Wupa Wastewater Treatment Plant, Abuja
(Sahel Journal of Life Sciences FUDMA (SAJOLS), 2025-02-02) Ibrahim Umar Faruok; Mujahid Musa; Omotosho Olalekan Azeez; Suleiman Kafilat Olayemi; Ifeoma Onyekwulunne Evlyn; Aina'u Musa Ajiya; Muhammad Umar; Aliyu Ibrahim Ahmed
Heavy metal pollution in water systems disrupts ecosystems and poses serious public health risks. Bioremediation remains the most cost-effective and eco-friendly, relying on naturally occurring microorganisms to degrade or transform toxic contaminants. This study was aimed at stimulating microbial isolates from Wupa Wastewater Treatment Plant (WWWTP) with nutrients to remove heavy metals from effluents obtained. Wastewater samples collected from Wupa WWTP were analyzed for physicochemical parameters, including pH, electrical conductivity, turbidity, temperature, BOD, COD, TSS, TDS, and concentrations of zinc (Zn), manganese (Mn), and iron (Fe), using standard methods. Bacteria and fungi were isolated microbiologically, and a nine-day container experiment was conducted with four nutrient setups containing different concentrations of peptone (150 ml, 75 ml) and glucose (9 g, 4.5 g) to enhance microbial degradation capability. Biomass growth was monitored every three days using a UV spectrophotometer. The results revealed that physicochemical parameters of the treated and untreated wastewater exhibit significant differences (p<0.05), except for pH and temperature. All physicochemical parameters showed significant differences (p < 0.05) between treated and untreated wastewater, except pH and temperature. Heavy metal concentrations also differed significantly (p < 0.05), with Mn decreasing from 0.37±0.20 mg/L (untreated) to 0.31±0.10 mg/L (treated), Fe from 1.58±0.2 mg/L to 0.91±0.3 mg/L, and Zn from 0.501±0.10 mg/L to 0.501±0.10 mg/L. Eight bacterial species were isolated. The biostimulated microbial consortium effectively remediated Mn, Fe, and Zn by 66.8%, 60.5%, and 70.2%, respectively. This study demonstrates promising potential for heavy metal removal from wastewater treatment plants and industrial effluents.
Investigation of Bacteria Biostimulation Strategy for Heavy Metal Bioremediation in Wupa Wastewater Treatment Plant, Abuja
(Sahel Journal of Life Sciences FUDMA (SAJOLS), 2025-02-02) Ibrahim Umar Faruok; Mujahid Musa; Omotosho Olalekan Azeez; Suleiman Kafilat Olayemi; Ifeoma Onyekwulunne Evlyn; Aina'u Musa Ajiya; Muhammad Umar; Aliyu Ibrahim Ahmed
Heavy metal pollution in water systems disrupts ecosystems and poses serious public health risks. Bioremediation remains the most cost-effective and eco-friendly, relying on naturally occurring microorganisms to degrade or transform toxic contaminants. This study was aimed at stimulating microbial isolates from Wupa Wastewater Treatment Plant (WWWTP) with nutrients to remove heavy metals from effluents obtained. Wastewater samples collected from Wupa WWTP were analyzed for physicochemical parameters, including pH, electrical conductivity, turbidity, temperature, BOD, COD, TSS, TDS, and concentrations of zinc (Zn), manganese (Mn), and iron (Fe), using standard methods. Bacteria and fungi were isolated microbiologically, and a nine-day container experiment was conducted with four nutrient setups containing different concentrations of peptone (150 ml, 75 ml) and glucose (9 g, 4.5 g) to enhance microbial degradation capability. Biomass growth was monitored every three days using a UV spectrophotometer. The results revealed that physicochemical parameters of the treated and untreated wastewater exhibit significant differences (p<0.05), except for pH and temperature. All physicochemical parameters showed significant differences (p < 0.05) between treated and untreated wastewater, except pH and temperature. Heavy metal concentrations also differed significantly (p < 0.05), with Mn decreasing from 0.37±0.20 mg/L (untreated) to 0.31±0.10 mg/L (treated), Fe from 1.58±0.2 mg/L to 0.91±0.3 mg/L, and Zn from 0.501±0.10 mg/L to 0.501±0.10 mg/L. Eight bacterial species were isolated. The biostimulated microbial consortium effectively remediated Mn, Fe, and Zn by 66.8%, 60.5%, and 70.2%, respectively. This study demonstrates promising potential for heavy metal removal from wastewater treatment plants and industrial effluents.
Prevalence, Molecular Characterization and Antibiotic Susceptibility Pattern of Enteropathogenic Escherichia coli (EPEC) Strains Associated with Pediatric Diarrhoea in Abuja, Nigeria
(International Research Journal of Gastroenterology and Hepatology, 2025-02-02) Suleiman Kafilat Olayemi; Kolo Ibrahim; Baba John; Maryam Auwal Cheldu
Aim: To determine the prevalence, molecular characteristics, and antibiotic susceptibility pattern of Enteropathogenic Escherichia coli (EPEC) strains isolated from children under five years of age presenting with acute diarrhoea in Abuja, Nigeria, and to evaluate the utility of combining serotyping with molecular diagnostics in EPEC identification. Study Design: Cross-sectional descriptive study. Place and Duration of Study: Abuja Municipal Area Council (AMAC), Federal Capital Territory, Abuja, Nigeria. Garki Hospital, Federal Medical Center, Abuja and National Hospital, Abuja between March 2022 and February 2023. Methodology: A total of 200 faecal samples were collected from pediatric patients (aged <5 years) with acute diarrhoea. E. coli was isolated using standard biochemical techniques. Confirmed isolates were serotyped with polyvalent and monovalent EPEC antisera. Molecular identification of the eaeA (intimin) and bfpA (bundle-forming pilus) virulence genes was carried out using polymerase chain reaction (PCR). Isolates were classified as typical EPEC (positive for both genes) or atypical EPEC (positive for eaeA only). Antimicrobial susceptibility testing was performed using the disc diffusion method for commonly used antibiotics. Results: Of the 200 stool samples analyzed, 19 (9.5%) were confirmed as EPEC. The most frequently detected serotype was O55:K59 (B5). Molecular analysis revealed that 5 isolates (26.3%) were positive for bfpA and 1 (5.2%) for both eaeA and bfpA genes, indicating a predominance of atypical EPEC strains. The remaining isolates lacked both virulence genes, suggesting the limitations of serotyping alone. Antimicrobial susceptibility testing showed 100% resistance to ampicillin, with high resistance to trimethoprim (89.5%) and tetracycline (84.2%). Ciprofloxacin, ceftazidime, and ceftriaxone exhibited the highest effectiveness. Multidrug resistance was observed in 26.3% of isolates. Conclusion: Atypical EPEC strains are more prevalent than typical EPEC in children with diarrhoea in Abuja. Combining molecular diagnostics with serotyping enhances detection accuracy. The high rate of antibiotic resistance, including multidrug resistance, underscores the need for routine surveillance to guide effective treatment and public health interventions.