Browsing by Author "Ezeagu Gloria Ginikanwa"

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Biodecolourization of Textile Effluents using Lignolytic Enzymes Produced from Selected Bacterial and Fungal Isolates from Waste Dump Site
(Nile University of Nigeria, 2023-02-02) Omotosho Olalekan Azeez; Ezeagu Gloria Ginikanwa; Sambo Datsugwai Mohammed Sani
Textile effluent contains enormous chemicals with detrimental environmental effects on both fauna and flora due to its chemical compositions. In this study, the effect of lignolytic enzymes produced by microorganisms for the treatment of textile effluent was examined using standard microbiological techniques. The potential of the isolates to produce laccase (L), lignin peroxidases (LiP) and manganase peroxidase (MnP) was investigated using streak plate method and assay methods. The L, LiP and MnP enzymes produced with the optimal processing parameter were used to decolorize textile effluent singly and as consortia for ten (10) days. Fourteen (14) microbial isolates which include eight (8) bacterial and four (4) fungi were isolated from soil contaminated with textile effluents. Aspergillus terreus and Aspergillus niger showed higher production of laccase with 8.0 mm diameter zone of inhibition. Bacillus licheniformis and Bacillus subtilis had the widest zone of inhibition (12.0 and 8.0 mm) respectively. Only Aspergillus flavus however had the potential to produce lignin peroxidase (with 10 mm zones of clearance) of all the fungi isolated in this study. Laccase recorded the highest decolourization (72.5%) comparable to 71.1% observed for the three-enzyme combination while LiP has 57.0%. This finding established the potential use of bacterial and fungal ligninocellulolytic enzymes for the decolourization of textile effluent.
Isolation And Identification of Indoor Air Microflora in Microbiology Laboratory of Nile University of Nigeria
(Nile University of Nigeria, 2023-02-02) Ezeagu Gloria Ginikanwa; Nasir Ja'Afar Zainab
A study was conducted to isolate and identify air microflora in Microbiology Laboratory of Nile University of Nigeria, Abuja. Nutrient agar and Potato Dextrose agar were prepared and poured into petri dishes and exposed to the air in Microbiology laboratory for 30minutes in the morning before the commencement of class activities and in the afternoon, during class activities. The plates were incubated at 37 ºC and 25 ºC respectively to isolate bacteria and fungi. The isolated bacteria species were Staphyloccocus aureus, Escherichia coli, Klebsiella sp. and Alkaligenes faecalis, while the fungal species were Aspergillus niger, Penicillium sp. Aspergillus fumigatus, Mucor flavus and Fusariummoniliforme. The microbial counts of the afternoon exposure were much higher than those of the morning hours and the isolated bacterial species demonstrated relatively high sensitivity to antibacterial antibiotics used for the study except for Klebsiella sp. which had 20 % resistance and Alcaligenes faecalis with 30 % resistance. It is recommended that all environmental conditions that can facilitate the proliferation of these microorganisms in the laboratory should be prevented in order to protect the health of staff and students.